The Rapid Determinaton of L-Leucine, L-Isoleucine and L-Valine in a Mixture by Infrared Spectrometry
نویسندگان
چکیده
منابع مشابه
Diagnosis of maple syrup urine disease by determination of L-valine, L-isoleucine, L-leucine and L-phenylalanine in neonatal blood spots by gas chromatography-mass spectrometry.
A novel method was developed for the diagnosis of maple syrup urine disease (MSUD) by the determination of L-valine, L-leucine, L-isoleucine and L-phenylalanine in dried blood spots of newborns by gas chromatography-mass spectrometry (GC-MS). The four amino acids were extracted from blood samples by methanol and derivatized by n-butanol and trifluroacetic anhydride under optimum reaction condit...
متن کاملDiauxie used as a microbiological assay for L-isoleucine or L-valine in Escherichia coli.
FIG. 1. Diauxic lag of cuiltures conitaining valine and isoleucine. All cultures were grown by shakinig (330 rev/ min) at 37 C in medium containing 2.0 g ofNaH2PO4 O H20, 7.0 g ofK2HP04, 2.0 g of (NH4)2SO4, 0.2 g of MgSO4 v 7H20 and I mg of thiamine hydrochloride in I liter of distilled water. Overnight cultures grown in 0.2%o glucose were harvested by centrifugation during exponential phase, r...
متن کاملStructural Basis for Double-Sieve Discrimination of L-Valine from L-Isoleucine and L-Threonine by the Complex of tRNAVal and Valyl-tRNA Synthetase
Valyl-tRNA synthetase (ValRS) strictly discriminates the cognate L-valine from the larger L-isoleucine and the isosteric L-threonine by the tRNA-dependent "double sieve" mechanism. In this study, we determined the 2.9 A crystal structure of a complex of Thermus thermophilus ValRS, tRNA(Val), and an analog of the Val-adenylate intermediate. The analog is bound in a pocket, where Pro(41) allows a...
متن کاملGlycyl dehydropeptides of leucine, valine, and isoleucine.
Glycyl dehydropeptides of alanine, phenylalanine, norvaline, norleucine, and or-aminobutyric acid, and N-acetyl dehydropeptides of these amino acids, tyrosine, valine, and leucine have been prepared (l-3). Although the glycyl dehydropeptides were found to be hydrolyzed by tissue extracts, the only enzymatically susceptible N-acetyl dehydropeptide was that of alanine (1). In an effort to acquire...
متن کاملA novel l-isoleucine-4′-dioxygenase and l-isoleucine dihydroxylation cascade in Pantoea ananatis
A unique operon structure has been identified in the genomes of several plant- and insect-associated bacteria. The distinguishing feature of this operon is the presence of tandem hilA and hilB genes encoding dioxygenases belonging to the PF13640 and PF10014 (BsmA) Pfam families, respectively. The genes encoding HilA and HilB from Pantoea ananatis AJ13355 were cloned and expressed in Escherichia...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1963
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.37.116